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Experimental procedures were performed in accordance with
the approved guidelines. The inclusion and exclusion criteria are shown in Supplementary
Figure S4. A total of 182 subjects were recruited prospectively to the study at Ehime University Hospital (Ehime,
Japan) between January 2012 and August 2015. Among 113
subjects diagnosed with fatty liver after ultrasonography during hospitalization for liver scrutiny, 85 subjects met the inclusion criteria,
including the absence of gastrointestinal or other disorders, and were enrolled as patients
with NAFLD. Three patients with NAFLD did not consent to liver biopsy.All biopsy samples were evaluated by a liver pathologist.
NASH was confirmed according to Matteoni’s classification, and 65 patients with
Matteoni’s types 3 and 4 were diagnosed with NASH47. After we explained the methods and objectives
of the study, 24 of the patients with NASH refused to participate, leaving a total of 41 NASH patients.
Among the 69 subjects who were not diagnosed with fatty liver using
ultrasonography during hospitalization for health checkup, 49 subjects met the inclusion criteria.Also visit my webpage – daily weight management supplement; Nelly,
